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Fig. 7 | Parasites & Vectors

Fig. 7

From: SjCa8, a calcium-binding protein from Schistosoma japonicum, inhibits cell migration and suppresses nitric oxide release of RAW264.7 macrophages

Fig. 7

Correlations between intracellular calcium levels and reduced NO generation by rSjCa8. After incubation with or without 1 μg/ml LPS, RAW264.7 macrophages were treated with 20 μg/ml rSjCa8 plus different reagents that can alter intercellular calcium level, as described in the methods section. Cells were loaded with the NO probe DAF-AM (green) and the calcium ion probe Rhod-2 AM (red). Green and red fluorescent intensities were detected simultaneously by laser-scanning capture microscopy (600×)

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