Fig. 1

The strategy for the construction of the chimeric inhibitors sequences using the construction of the inhibitor boophilin domain 1 (D1) in pPICZαB plasmid as a template. Nucleotides sequence of wild D1, the region used to construct internal oligonucleotides is represented by left right arrow. Sequence nucleotides and amino acids in region P1-P4’ of the D1 inhibitor that was replaced in production of mutants are shaded in gray. AOX5’ is the primer forward present in plasmid pPICZαB and AOX3’ the reverse primer. The internal primer to construct the mutants were designer in the same place, the internal reverse primer is matched with AO5’ in PCR reaction to form the first part of the inhibitor and internal forward primer is matched with AOX3’ to form the second part of the inhibitor, this parts were joined by a PCR reaction using the AOX5’and AOX3’ primers