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Fig. 3 | Parasites & Vectors

Fig. 3

From: Production of serine protease inhibitors by mutagenesis and their effects on the mortality of Aedes aegypti L. larvae

Fig. 3

Purification of the chimeras T6/D1, T149/D1, T23/D1 and D1 wild expressed in P. pastoris by ion exchange chromatography in HiTrap Q column. The supernatant of the culture medium dialyzed with 0.05 M Tris/HCl pH 8.0 buffer was applied in the column pre equilibrated with the same buffer. The elution of proteins was performed with a linear gradient of NaCl (0–1 M). The regions containing inhibitory activities are indicated by gray arrows

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