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Fig. 4 | Parasites & Vectors

Fig. 4

From: Production of serine protease inhibitors by mutagenesis and their effects on the mortality of Aedes aegypti L. larvae

Fig. 4

Characterization of the mutants: T6/D1, T23/D1, T149/D1 and D1 wild. a SDS–PAGE (15 %) of the flow through of the ion exchange chromatography in HiTrap Q column of the mutants T6/D1, T23/D1, T149/D1 and D1 wild. b Inhibition assay of the chimeric inhibitors for larvae serine proteases. Enzymatic activities using the L4 midgut extract of Ae. aegypti and chromogenic substrate for trypsin (Tosyl-Gly-Pro-Arg-pNA), chymotrypsin (Suc-Ala-Ala-Pro-Phe-pNA) and elastase (Suc-Ala-Ala-Pro-Val-pNA). Both trypsin and chymotrypsin assays were performed using 0.8 mg total protein, and 24.5 mg total protein for elastase

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Parasites & Vectors

ISSN: 1756-3305

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