Erratum to: Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

Webster, B. L.; Rabone, M.; Pennance, T.; Emery, A. M.; Allan, F.; Gouvras, A.; Knopp, S.; Garba, A.; Hamidou, A. A.; Mohammed, K. A.; Ame, S. M.; Rollinson, D.; Webster, J. P.

doi:10.1186/s13071-015-1134-5

Table 1 Details of the 18 selected microsatellite loci and the characteristics of the two multiplex microsatellite PCR assays. Loci Sh1-15 are from Travis et al., 2013 and Loci C102, C111 and C131 are from Gower et al., 2011. For Niger Ho = 0.596, He = 0.609, for Pemba Ho = 0.599, He = 0.638. The overall Ho = 0.597, He = 0.623

From: Erratum to: Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

Panel 1	Marker	Forward Primer 5'- 3'	Reverse Primer 5'- 3'	Dye	Size Range (bp)	Repeat	A	Niger		Zanzibar
	Marker	Forward Primer 5'- 3'	Reverse Primer 5'- 3'	Dye	Size Range (bp)	Repeat	A	H _o	H _e	H _o	H _e
Panel 1	C102	TGTCTCTGTGAATGACCGAAT	TTAGATGAATAATAATGTTGAAACCAC	VIC	184-199	ATT	6	0.42	0.37	0.02	0.02
	Sh1	GCATCCAATTTCGTACAC	CCACATTAGGCCAACAAG	VIC	245-284	AAT	13	0.76	0.72	0.84	0.80
	Sh14	GTCCTCCTTCCCTCTTTG	CACATTCGTCCTAGATATCG	NED	184-240	ACTC	15	0.94	0.85	0.86	0.88
	C131	CTTGTCATTTGGGCATTGTG	CATGGTGAGGTTCAAACGTG	NED	253-265	AAT	4	0.00	0.00	0.00	0.00
	Sh6	GGTGGATTACGCAATAG	TTTAATCAACCGGGTGTC	NED	309-321	AAT	7	0.48	0.44	0.84	0.76
	Sh9	GGGATGTATGCAGACTTG	TTGTTTGGCTGCAGTAAC	6-FAM	197-227	AAT	11	0.46	0.76	0.46	0.86
	Sh3	GCTGAGCTTGAGATTG	CTTCTGTCCCATCGATACC	6-FAM	270-366	AAT	30	0.76	0.86	0.94	0.86
	C111	CCCTTGTCTTCAATGCGTTA	GAACGTCTAACTGGCGATCA	PET	201-225	ATT	9	0.74	0.67	0.76	0.68
	Sh7	TCCAAGCACCATTATCAAG	ACGGAAACTTGTTGAAATG	PET	293-311	AAT	7	0.46	0.62	0.42	0.48
Panel 2	Sh2	TTAGTGTGTTTGGCTTCAAC	CCTCGAATGAAATCCTGAC	NED	155-218	AAT	21	0.84	0.90	0.56	0.89
	Sh5	TGTGCACAAGAAAGATTAAATG	ACGACAATGTTGCAAGTTC	NED	263-314	AAT	16	0.78	0.81	0.36	0.48
	Sh13	GAGCAGCTATTTCGTATCG	ACCGTGGACAGTTCATCAG	6-FAM	163-211	AAT	17	0.78	0.72	0.68	0.64
	Sh4	CCCATCGCTGATATTAAAG	TCTAGTCGTCTTGGGATCC	6-FAM	268-313	AAT	13	0.84	0.78	0.72	0.79
	Sh10	CGCATGTCATACCTATCTCC	GCTTATCAGGCCTATCTCC	PET	183-207	AAT	9	0.18	0.34	0.74	0.70
	Sh12	CGTCTTAGTGAGCCAGATG	CTCGTGGACATCATCAG	PET	245-278	AAC	11	0.06	0.06	0.56	0.65
	Sh8	CTAAACTGGCAAGATTTC	CAACGTGCCTTTATTTC	PET	282-321	AAT	14	0.76	0.81	0.84	0.83
	Sh11	TTGGTTTAGAAATTACATCACC	CCAACAATATTAATGGACAGC	VIC	183-213	ATC	9	0.68	0.58	0.68	0.69
	Sh15	CTTTCAGTAGGATTTGTTG	CGACGTCAAGCACTGTAC	VIC	274-301	ATC	10	0.78	0.65	0.50	0.466

Panel = single mulitplex PCR. A = observed number of alleles. Dye = the fluorescent dye label of the forward primer (VIC = green, NED = yellow, 6-FAM = Blue, PET = red). Ho = observed heterozygosity, He = expected heterozygosity

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ISSN: 1756-3305

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