Table 2 Proteins identified in the midgut from Ornitodoros erraticus females before feeding (unfed group) and after 48 h post-feeding (fed group) involved in the following biological activities and process: Peptidase activity, iron metabolism and transport, oxidoreductase, protein folding and response to stress and endocytosis, membrane traffic and protein transport

Unfed, Fed

Q2WFX6

AP

Aspartic protease

Pa1

Aspartic-type endopeptidase

2

2

0.32

0.32

Unfed, Fed

E7E820

-

Cathepsin D2

Pa2

Aspartic-type endopeptidase

2

3

0.34

0.26

Unfed, Fed

B7P6S9

IscW_ISCW000202

Tick legumain

Pa3

Cysteine-type endopeptidase

2

2

0.17

0.17

Unfed, Fed

F0J8F6

-

Metallopeptidase (Fragment)

Pa4

-

2

3

0.22

0.41

Unfed, Fed

B7PA58

IscW_ISCW003100

Putative uncharacterized protein

Pa5

Metalloexopeptidase

2

2

0.14

0.14

Unfed, Fed

Q6U8A8

-

Serine protease-like protein

Pa6

Serine-type endopeptidase

4

4

0.96

0.75

Unfed, Fed

Q09JL3

-

Signal peptidase complexI

Pa7

Serine-type peptidase

5

2

1.28

0.39

Unfed

E2BXE8

EAI_04817

AFG3-like protein 2

Pa8

Metalloendopeptidase

4

-

0.51

-

Unfed

B7Q203

IscW_ISCW009180

ATPase

Pa9

Metalloendopeptidase

3

-

0.26

-

Unfed

B7P573

IscW_ISCW001592

Processing peptidase beta subunit,

Pa10

Metalloendopeptidase

2

-

0.13

-

Unfed

B4NAG0

Dwil\GK11711

GK11711

Pa11

Peptidase activity

2

-

0.36

-

Unfed

B7PXW8

IscW_ISCW020703

Signal peptidase complex, subunit SPC25

Pa12

Peptidase activity

2

-

0.31

-

Fed

B7Q579

IscW_ISCW021356

Acylamino-acid-releasing enzyme

Pa13

Serine-type peptidase

-

2

-

0.08

Fed

M7CBK1

UY3_00674

Protein DDI1 like protein 2

Pa14

Aspartic-type endopeptidase

-

2

-

0.06

Fed

A0A087UYK2

X975_16479

Signal peptidase complex catalytic subunit SEC11C

Pa15

Serine-type peptidase

-

2

-

0.21

Unfed, Fed

B9UNL8

-

Aconitate/iron-regulatory protein

Ir1

4 iron, 4 sulfur cluster binding

2

3

0.3

0.1

Unfed, Fed

B7P592

IscW_ISCW015613

Amidophosphoribosyltransferase (ATase)

Ir2

Iron-sulfur cluster binding

2

3

0.24

0.19

Unfed, Fed

O99806

-

Cytochrome c oxidase subunit 1

Ir3

Iron ion binding

2

2

0.12

0.38

Unfed, Fed

A6N9Q6

-

Ferritin

Ir4

Iron ion transport

2

2

0.36

0.36

Unfed

Q16LR5

AAEL012552

AAEL012552-PA

Ir5

Iron-sulfur cluster binding

3

-

0.13

-

Unfed

J3JUT5

YQE_06758

Cytochrome b-c1 complex subunit Rieske

Ir6

2 iron, 2 sulfur cluster binding

2

-

0.24

-

Unfed

F4WQE0

G5I_08058

NADH dehydrogenase [ubiquinone] iron-sulfur protein

Ir7

4 iron, 4 sulfur cluster binding

2

-

0.2

-

Unfed

B7PNX4

IscW_ISCW005985

NADH:ubiquinone oxidoreductase, NDUFV1/51 kDa subunit,

Ir8

4 iron, 4 sulfur cluster binding

2

-

0.13

-

Unfed

B7PBH7

IscW_ISCW003299

NADH-ubiquinone reductase

Ir9

Iron-sulfur cluster binding

2

-

0.22

-

Unfed

A7RKR4

v1g179073

Predicted protein

Ir10

4 iron, 4 sulfur cluster binding

3

-

0.37

-

Unfed

Q86GF8

-

Putative uncharacterized protein

Ir11

4 iron, 4 sulfur cluster binding

2

-

0.08

-

Unfed

G6D2B9

KGM_16016

Uncharacterized protein

Ir12

4 iron, 4 sulfur cluster binding

3

-

0.38

-

Unfed

T1FN77

HELRODRAFT_185731

Uncharacterized protein

Ir13

Iron-sulfur cluster binding

3

-

0.13

-

Unfed, Fed

A1KXI6

-

Blo t aldehyde dehydrogenase allergen

Ox1

Oxidoreductase

2

3

0.44

0.49

Unfed, Fed

B7P4E1

IscW_ISCW000393

Glutamate dehydrogenase

Ox2

Oxidoreductase

3

3

0.17

0.28

Unfed, Fed

B7QKE4

IscW_ISCW023707

Glycerol-3-phosphate dehydrogenase

Ox3

Oxidoreductase

2

2

0.08

0.18

Unfed, Fed

Q4PLZ0

-

Mitochondrial malate dehydrogenase

Ox4

Oxidoreductase

2

6

0.35

1.83

Unfed, Fed

Q09JE3

-

Superoxide dismutase [Cu-Zn]

Ox5

Oxidoreductase

3

3

1.87

1.36

Unfed, Fed

A6N9S1

-

Thioredoxin peroxidase

Ox6

Oxidoreductase

6

3

1.92

0.54

Unfed, Fed

A6NA14

-

Truncated peroxiredoxin

Ox7

Oxidoreductase

3

3

0.55

0.55

Unfed, Fed

F0J8S6

-

FKBP-type peptidyl-prolyl cis-trans isomerase

Ox8

Protein folding

3

2

0.35

0.26

Unfed, Fed

B2ZWT4

CyPA

Peptidyl-prolyl cis-trans isomerase

Ox9

Protein folding

3

3

0.84

0.84

Unfed, Fed

A0A087UTZ9

X975_09861

T-complex protein 1 subunit alpha

Ox10

Protein folding

2

3

0.11

0.4

Unfed, Fed

E9GYM5

DAPPUDRAFT_306806

T-complex protein 1 subunit gamma

Ox11

Protein folding

3

3

0.17

0.33

Unfed, Fed

F0J8P3

-

HSP70 family member

Ox12

Protein folding/response to stress

12

11

4.0

3.5

Unfed, Fed

B7QI01

IscW_ISCW014265

Hsp90 protein

Ox13

Protein folding/response to stress

16

21

1.17

3.03

Unfed, Fed

B7QC85

IscW_ISCW022766

Tumor rejection antigen (Gp96),

Ox14

Protein folding/response to stress

5

9

0.95

1.46

Unfed, Fed

Q2YFF0

-

Glutathione transferase mu class

Ox15

Response to stress and detoxification

2

2

0.27

0.25

Unfed

B7P0P1

IscW_ISCW016225

DNA topoisomerase 2

Ox16

Oxidoreductase

2

-

0.38

-

Unfed

B7QBE5

IscW_ISCW013475

Lipophorin receptor

Ox17

Oxidoreductase

2

-

0.27

-

Unfed

Q2XW18

PHGPX

Glutathione peroxidase

Ox18

Response to oxidative stress

4

-

1.12

-

Fed

A0A087U1A2

X975_14796

Copper chaperone for superoxide dismutase

Ox19

Oxidation-reduction process

-

2

-

0.24

Fed

Q6DJQ3

aldh9a1 TEgg018l09.1-001

Aldehyde dehydrogenase 9 family, member A1

Ox20

Oxidoreductase

-

2

-

0.12

Fed

B7Q8W6

IscW_ISCW010532

Alkyl hydroperoxide reductase, thiol specific antioxidant

Ox21

Oxidoreductase

-

2

-

0.27

Fed

U6PA25

HCOI_01121300

Endonuclease exonuclease phosphatase

Ox22

Oxidoreductase

-

2

-

0.07

Fed

I1WDI0

-

Putative 17 beta-hydroxysteroid dehydrogenase

Ox23

Oxidoreductase

-

2

-

0.34

Fed

E9HGS0

DAPPUDRAFT_228809

Uncharacterized protein

Ox24

Oxidoreductase

-

2

-

0.22

Fed

T1FVK5

HELRODRAFT_194011

Uncharacterized protein

Ox25

Oxidoreductase

-

2

-

0.12

Fed

B7QJ21

IscW_ISCW023397

Chaperonin complex component, TCP-1 eta subunit,

Ox26

Protein folding

-

2

-

0.21

Fed

Q0ZBX3

AG-0383 F-Gp

Putative accessory gland protein

Ox27

Protein folding

-

2

-

0.24

Fed

A0A067RDC2

L798_09307

T-complex protein 1 subunit delta

Ox28

Protein folding

-

2

-

0.37

Fed

F0J987

-

Endoplasmic reticulum glucose-regulated protein

Ox29

Protein folding/response to stress

-

4

-

0.67

Unfed, Fed

B7QHS0

IscW_ISCW015012

Flotillin

Et1

_

4

2

0.33

0.14

Unfed, Fed

B7P427

IscW_ISCW001550

Transmembrane protein Tmp21

Et2

Protein transport

2

2

0.48

0.30

Unfed, Fed

B7P6P0

IscW_ISCW001001

Glycoprotein 25 l

Et3

Protein transport

3

3

0.63

0.44

Unfed, Fed

B7PUK8

IscW_ISCW019441

Clathrin heavy chain

Et4

Vesicle-mediated transport

12

10

0.25

0.21

Unfed, Fed

B7PQE0

IscW_ISCW006283

Coatomer, alpha chain

Et5

Vesicle-mediated transport

2

2

0.06

0.06

Unfed, Fed

B7PDY5

IscW_ISCW004922

Vesicle coat complex COPII, GTPase subunit SAR1

Et6

Vesicle-mediated transport

3

3

0.53

0.60

Unfed

B7Q4N0

IscW_ISCW011849

Cargo transport protein EMP24

Et7

Protein transport

2

-

0.22

-

Unfed

B7PGX4

IscW_ISCW003940

Synaptic vesicle-associated integral membrane protein

Et8

Vesicle-mediated transport

4

-

0.65

-

Fed

B7QNW0

IscW_ISCW015531

Protein required for fusion of vesicles in vesicular transport, alpha-SNAP

Et9

Protein transport

-

3

-

0.35

Fed

B7PYR6

IscW_ISCW020077

Protein transport protein SEC61 alpha subunit

Et10

Protein transport

-

4

-

0.32

Fed

B7P806

IscW_ISCW016976

Vesicle docking protein P115

Et11

Protein transport

-

2

-

0.08

Fed

B7Q6V9

IscW_ISCW011108

AP-2 complex subunit beta-1

Et12

Vesicle-mediated transport

-

5

-

0.17

Fed

B7QAA7

IscW_ISCW012850

Coatomer beta subunit

Et13

Vesicle-mediated transport

-

3

-

0.12

Fed

B7QCH5

IscW_ISCW022475

Coatomer gamma subunit

Et14

Vesicle-mediated transport

-

3

-

0.14

Fed

B7Q5G4

IscW_ISCW021581

Endocytosis/signaling protein EHD1

Et15

Vesicle-mediated transport

-

2

-

0.18