Fig. 4

Inhibitory effects of cecropin-TY1 on LPS-stimulated inflammatory response signal pathway in peritoneal macrophages. a–b Effects of cecropin-TY1 on (a) MAPKs and (b) NF-κB activation in peritoneal macrophages after 100 ng/mL LPS stimulation. The photograph is a representative one of three independent experiments. Peritoneal macrophages were stimulated with or without LPS (100 ng/mL), then different concentrations of cecropin-TY1 (cec-TY1, 5, 10, 20 μg/mL) were added immediately and incubated for 30 min, and macrophages were lysed for western blot analysis. (c–i Ratio of (c) P-ERK1 (42 kDa), (d) P-ERK2 (44 kDa), (e) P-JNK1 (46 kDa), (f) P-JNK2 (54 kDa), (g) P-p38, (h) P-IκBα and (i) P-p65 to β-actin. Band densities were analyzed using Quantity One software (Bio-Rad, Richmond, CA USA). Data were presented as mean ± SEM. *P <0.05, **P < 0.01, ratios of peptide-treated groups are significantly different from that induced by 100 ng/mL LPS alone