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Fig. 1 | Parasites & Vectors

Fig. 1

From: Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies

Fig. 1

a - Profiles obtained by DNA genotyping of Trypanosoma cruzi samples isolated from patients of the Jequitinhonha Valley, Minas Gerais, Brazil, resultant from the amplification of the 3' region of the 24Sα rDNA gene. MW - Molecular weight of 100 bp, Br - negative reaction control, TcI - representative clone of TcI lineage (P209 cl1, 110 bp), TcIV - representative clone TcIV lineage (CANIII, 120 bp), TcII - representative clone of TcII lineage (MAS cl1, 125 bp), TcIII - representative clone of the TcIII lineage (CM-17, 110 bp), TcVI - representative clone of the hybrid lineage TcVI (Tulahuen cl2, 125 bp) and TcV - representative clone of TcV lineage (Bug2148, 110 bp + 125 bp). A1 – sample 229, A2 – sample 452, A3 – sample 748, A4 - sample 798, A5 –sample 205, A6 – sample 1918, A7 –sample 2535, A8 –sample 2491, A9 –sample 264, A10 – sample 376, A11 – sample 646 and A12 – sample 211. b - Profiles obtained with DNA of Trypanosoma cruzi samples isolated from chagasic patients of the Jequitinhonha Valley, Minas Gerais, Brazil, by the analysis of the HSP60 gene polymorphism after PCR-RFLP. MW - Molecular weight of 100 bp, A1 – sample 1918, A2 – sample PSF060, A3 – sample 438, A4 – sample 543, A5 – sample 229, A6 – sample 2119. Br – PCR negative control; TcI – TcVI samples profile of the reference clones of the 6 lineages of T. cruzi (TcI - P209 cl1, single band of 432 – 462 bp; TcII – MAS cl1, single band of 432 – 462 bp; TcIII – CM-17 double bands; TcIV – CAN III cl1, single band; TcV – Bug 2148, triple bands; TcVI – Tulahuen cl2, triple bands). c - Profiles of DNA genotyping of T. cruzi samples isolated from chagasic patients of the Jequitinhonha Valley, Minas Gerais, Brazil, by the analysis of GPI/HhaI gene polymorphism via PCR-RFLP. MW - Molecular weight of 100 bp, BR – PCR negative control, A1 – sample 2014, A2 – sample 646, A3 – sample 453, A4 – sample 2119, A5 – sample 440, A6 – sample 501, A7 – sample 523, A8 – sample 2478, A9 – sample PSF060, A10 – sample 1100, A11 – sample 820, and A12– sample 2535.TcII – (clone TU18 cl1 representative of DTU TcII), TcV – (clone SO3 cl5, DTU TcV), TcI – (clone 92101601P cl1, DTU TcI), TcIII – (Clone X109/2, DTU TcIII), TcVI – (clone Tulahuen, DTU TcVI) and TcIV – (CANIII cl1 DTU TcIV)

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