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Fig. 2 | Parasites & Vectors

Fig. 2

From: Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies

Fig. 2

a – Profiles obtained by DNA genotyping of Trypanosoma cruzi isolated from patients of the Jequitinhonha Valley, Minas Gerais, Brazil, of the region 3' of the 24Sα rDNA gene. MW - Molecular weight of 100 bp, Br – PCR negative control. TcI – TcI representative clone (P209 cl1 profile of 110 bp), TcIV – representative clone of TcIV (CANIII, 120 bp), TcII - representative clone of TcII(MAS cl1, 125 bp), TcIII – representative clone of the lineage TcIII (CM-17, 110 bp), TcVI – representative of the hybrid lineage TcVI (Tulahuen cl2, 125 bp) and TcV – representative clone of lineage TcV (Bug2148, 110 bp + 125 bp); A1 – sample 229, A2 – sample 452, A3 – sample 748, A4 – sample 798, A5 – sample 1205, A6 – sample 1918, A7 – sample 2535, A8 –sample 2491, A9 – sample 264, A10 – sample 376, A11 – sample 646 and A12 – sample 2118. b - Profiles obtained by DNA genotyping of Trypanosoma cruzi isolated from patients of the Jequitinhonha Valley, Minas Gerais, Brazil, by analysis of polymorphism of subunit II of the cytochrome oxidase gene (COII). MW - Molecular weight of 1 kb, BR – PCR negative control, TcI – profile of bands of clone P209 cl1 (mitochondrial haplotype A - DTU TcI, Freitas et al., 2006), TcII – Tu18 cl2 (haplotype C - DTU TcII), TcVI –M6241 CL6 and C4 – Tulahuen cl2 (mitochondrial haplotype B - DTU TcIII and DTU TcVI); A1 – sample 452, A2 – sample 2535, A3 – sample 376, A4 – sample 229, A5 – 2408 sample, A6 – sample 264, A7 – sample 748, A8 – 1918 sample, A9 – sample 2491, A10 – sample 798, A11 – sample 501, A12 – sample 2118. c- Profiles obtained by DNA genotyping of Trypanosoma cruzi isolated from patients of the Jequitinhonha Valley, Minas Gerais, Brazil, by analysis of polymorphism of the intergenic spacer of the miniexon. MW - Molecular weight standard 1 kb; C1 – clone Agouti cl1 (DTU TcI), C2 – clone Tu18cl1 (DTU TcII), C3 and C4 – clones X109 2 (DTU TcIII) and 9212210R2 (DTU TcIV), C5 and C6 – clones SO3cl5 and Tula cl2 representatives of DTU (s) TcV and TcVI, respectively; A1 – sample 229, A2 – sample 452, A3 – sample 748, A4 – sample 798, A5 – sample 1205, A6 – sample 1337, A7 – sample 2118, A8 – sample 2119; A9 – sample 376, A10 – sample 2491, A11 – sample 493; A12 – sample 820, A13 – sample 1107; A14 – sample 2478; A15 – sample 1918; A16 – sample 2014, Br: PCR negative control

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