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Table 1 Genotyping of Trypanosoma cruzi isolates into DTUs (TcI – TcVI) according to the methodologies of [24] and [16]

From: Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies

T. cruzi DTU 24sα rDNA Souto et al. (1996) RFLP-HSP60 Sturm et al.(2003) RFLP-GPI Westenberger et al. (2005) RFLP-COXII Freitas et al. (2006) SL-IR Burgos et al. (2007)
DNA fragments in base pairs and the number of bands expected
TcI 110 bp 1 band 2 bands Haplotype A (262 bp + 81 bp + 30 bp) ~150/157 bp
TcII 125 bp 1 band 3 bands Haplotype C (212 bp + 81 bp) ~150/157 bp
TcIII 110 bp 2 bands 2 bands Haplotype B (294 bp + 81 bp) 200 bp
TcIV ~120 bp 1 band 3 bands Haplotype B (294 bp + 81 bp) 200 bp
TcV 110 bp + 125 bp 3 bands 4 bands Haplotype B (294 bp + 81 bp) ~150/157 bp
TcVI 125 bp 3 bands 4 bands Haplotype B (294 bp + 81 bp) ~150/157 bp