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Table 1 Genotyping of Trypanosoma cruzi isolates into DTUs (TcI – TcVI) according to the methodologies of [24] and [16]

From: Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies

T. cruzi

DTU

24sα rDNA

Souto et al. (1996)

RFLP-HSP60

Sturm et al.(2003)

RFLP-GPI

Westenberger et al. (2005)

RFLP-COXII

Freitas et al. (2006)

SL-IR

Burgos et al. (2007)

DNA fragments in base pairs and the number of bands expected

TcI

110 bp

1 band

2 bands

Haplotype A (262 bp + 81 bp + 30 bp)

~150/157 bp

TcII

125 bp

1 band

3 bands

Haplotype C (212 bp + 81 bp)

~150/157 bp

TcIII

110 bp

2 bands

2 bands

Haplotype B (294 bp + 81 bp)

200 bp

TcIV

~120 bp

1 band

3 bands

Haplotype B (294 bp + 81 bp)

200 bp

TcV

110 bp + 125 bp

3 bands

4 bands

Haplotype B (294 bp + 81 bp)

~150/157 bp

TcVI

125 bp

3 bands

4 bands

Haplotype B (294 bp + 81 bp)

~150/157 bp