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Table 1 PCR conditions for identification of Candidatus Neoehrlichia used in this study

From: Candidatus Neoehrlichia sp. in an Austrian fox is distinct from Candidatus Neoehrlichia mikurensis, but closer related to Candidatus Neoehrlichia lotoris

Specifity Genetic marker Sequences of primer (5’- 3’) Annealing temperature (°C) Amplicon size (bp) References
Anaplasmataceae 16S EHR16SD: GGT ACC YAC AGA AGA AGT CC 54 345 [9]
EHR16SR: TAG CAC TCA TCG TTT ACA GC
Candidatus Neoehrlichia groEL NeoeGroELFw: CAG GTG AAG CAC TAG ATA AGT CCA 54 806 This study
NeoeGroELRv: ACA GCA GCA ACA TGC AAT CCA
Candidatus Neoehrlichia 16S 16SCNM_for: GTG GCA GAC GGG TGA GTA AT 60 1,053 This study
16SCNM_rev: TGC AGC ACC TGT GTA AGG TC