Table 1 PCR conditions for identification of Candidatus Neoehrlichia used in this study
Specifity | Genetic marker | Sequences of primer (5’- 3’) | Annealing temperature (°C) | Amplicon size (bp) | References |
|---|---|---|---|---|---|
Anaplasmataceae | 16S | EHR16SD: GGT ACC YAC AGA AGA AGT CC | 54 | 345 | [9] |
EHR16SR: TAG CAC TCA TCG TTT ACA GC | |||||
Candidatus Neoehrlichia | groEL | NeoeGroELFw: CAG GTG AAG CAC TAG ATA AGT CCA | 54 | 806 | This study |
NeoeGroELRv: ACA GCA GCA ACA TGC AAT CCA | |||||
Candidatus Neoehrlichia | 16S | 16SCNM_for: GTG GCA GAC GGG TGA GTA AT | 60 | 1,053 | This study |
16SCNM_rev: TGC AGC ACC TGT GTA AGG TC |