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Fig. 3 | Parasites & Vectors

Fig. 3

From: Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4

Fig. 3

TLR4, MyD88 and NF-κB p65 protein expression in primary mouse intrahepatic biliary epithelial cells (MIBECs). MIBECs were pre-treated with control peptide group and TLR4 inhibitor (VIPER) group and stimulated by medium (negative control), LPS, excretory/secretory products of C. sinensis (ESP) and LPS + ESP (L + E) for 48 h, respectively. Each value represents the mean ± SE of three or more independent experiments. (a) The gel images of TLR4, MyD88 and NF-κB p65 showed by western blot .The relative expression of TLR4 (b), MyD88 (c) and NF-κB p65 (d) in each group. *represents significant differences (P < 0.05) were found, compared with the negative groups; #represents significant differences (P < 0.05) were found, compared with the indicated group

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