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Table 2 Ranking orders of the reference genes of C. megacephala in different experimental conditions

From: Reference gene stability of a synanthropic fly, Chrysomya megacephala

Conditions   ΔCt Bestkeeper NormFinder geNorm
Rank Gene Stability Gene Stability Gene Stability Gene Stability
Developmental 1 EF1 2.597 18S 1.758 Rps7 1.384 EF1| 1.550
Rps7
Stages 2 Rps7 2.650 Rpl8 2.351 EF1 1.392   
  3 α-TUB 2.702 Rps7 3.169 α-TUB 1.455 α-TUB 1.655
  4 β-TUB 2.840 β-TUB 3.191 β-TUB 1.532 Rpl8 1.975
  5 Rpl8 2.849 α-TUB 3.327 Rpl8 1.592 β-TUB 2.179
  6 Actin 3.266 Actin 3.395 Actin 2.357 GAPDH 2.406
  7 GAPDH 3.296 TBP 3.899 GAPDH 2.575 Actin 2.549
  8 TBP 3.383 EF1 3.904 TBP 2.645 TBP 2.680
  9 18S 4.828 GAPDH 4.664 18S 4.483 18S 3.157
Food 1 EF1 1.941 18S 0.449 EF1 0.874 Actin| 0.766
GAPDH
Resources 2 18S 2.122 Rps7 1.090 18S 1.118   
  3 GAPDH 2.127 TBP 1.582 GAPDH 1.512 EF1 1.060
  4 Actin 2.260 EF1 1.687 β-TUB 1.548 Rpl8 1.221
  5 Rpl8 2.276 α-TUB 1.766 Rpl8 1.639 18S 1.603
  6 β-TUB 2.307 β-TUB 1.860 Actin 1.730 β-TUB 1.796
  7 α-TUB 2.570 GAPDH 2.077 α-TUB 1.983 α-TUB 2.025
  8 TBP 2.685 Rpl8 2.090 TBP 2.139 TBP 2.182
  9 Rps7 3.016 Actin 2.338 Rps7 2.602 Rps7 2.367
Different 1 Rpl8 1.194 18S 0.477 18S 0.659 Rpl8| 0.567
EF1
Drugs 2 α-TUB 1.200 β-TUB 0.637 α-TUB 0.668   
  3 18S 1.238 TBP 0.839 TBP 0.758 Actin 0.705
  4 GAPDH 1.239 GAPDH 0.948 Rpl8 0.785 GAPDH 0.789
  5 TBP 1.246 Actin 0.980 GAPDH 0.798 α-TUB 0.977
  6 Actin 1.295 Rpl8 1.057 Actin 0.918 TBP 1.054
  7 β-TUB 1.483 α-TUB 1.071 β-TUB 1.178 18S 1.113
  8 EF1 1.507 EF1 1.094 EF1 1.314 β-TUB 1.229
  9 Rps7 1.800 Rps7 1.184 Rps7 1.646 Rps7 1.356
Different 1 GAPDH 0.604 β-TUB 0.233 GAPDH 0.212 α-TUB| 0.260
β-TUB
Heavy metals 2 α-TUB 0.611 GAPDH 0.269 α-TUB 0.297   
  3 β-TUB 0.636 α-TUB 0.338 β-TUB 0.351 GAPDH 0.336
  4 Rpl8 0.691 Rpl8 0.408 Rpl8 0.423 Rpl8 0.411
  5 Actin 0.728 Actin 0.462 Actin 0.476 Actin 0.491
  6 Rps7 0.744 Rps7 0.528 Rps7 0.556 Rps7 0.577
  7 EF1 0.790 EF1 0.587 EF1 0.607 EF1 0.618
  8 18S 0.841 TBP 0.613 18S 0.705 18S 0.650
  9 TBP 1.095 18S 0.655 TBP 1.015 TBP 0.749
Different 1 TBP 1.232 EF1 0.523 EF1 0.059 EF1| 0.272
TBP
Tissues 2 EF1 1.269 TBP 0.558 TBP 0.136   
  3 Rpl8 1.306 α-TUB 0.634 Rpl8 0.221 Rpl8 0.428
  4 Rps7 1.412 Actin 0.749 Rps7 0.401 Rps7 0.639
  5 GAPDH 1.532 β-TUB 0.760 GAPDH 0.715 GAPDH 0.787
  6 α-TUB 1.572 Rps7 0.863 β-TUB 1.190 β-TUB 0.932
  7 β-TUB 1.753 Rpl8 0.887 α-TUB 1.477 α-TUB 1.026
  8 Actin 2.001 GAPDH 0.957 Actin 1.590 Actin 1.193
  9 18S 3.729 18S 3.714 18S 3.655 18S 1.756