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Table 2 Ranking orders of the reference genes of C. megacephala in different experimental conditions

From: Reference gene stability of a synanthropic fly, Chrysomya megacephala

Conditions

 

ΔCt

Bestkeeper

NormFinder

geNorm

Rank

Gene

Stability

Gene

Stability

Gene

Stability

Gene

Stability

Developmental

1

EF1

2.597

18S

1.758

Rps7

1.384

EF1|

1.550

Rps7

Stages

2

Rps7

2.650

Rpl8

2.351

EF1

1.392

  
 

3

α-TUB

2.702

Rps7

3.169

α-TUB

1.455

α-TUB

1.655

 

4

β-TUB

2.840

β-TUB

3.191

β-TUB

1.532

Rpl8

1.975

 

5

Rpl8

2.849

α-TUB

3.327

Rpl8

1.592

β-TUB

2.179

 

6

Actin

3.266

Actin

3.395

Actin

2.357

GAPDH

2.406

 

7

GAPDH

3.296

TBP

3.899

GAPDH

2.575

Actin

2.549

 

8

TBP

3.383

EF1

3.904

TBP

2.645

TBP

2.680

 

9

18S

4.828

GAPDH

4.664

18S

4.483

18S

3.157

Food

1

EF1

1.941

18S

0.449

EF1

0.874

Actin|

0.766

GAPDH

Resources

2

18S

2.122

Rps7

1.090

18S

1.118

  
 

3

GAPDH

2.127

TBP

1.582

GAPDH

1.512

EF1

1.060

 

4

Actin

2.260

EF1

1.687

β-TUB

1.548

Rpl8

1.221

 

5

Rpl8

2.276

α-TUB

1.766

Rpl8

1.639

18S

1.603

 

6

β-TUB

2.307

β-TUB

1.860

Actin

1.730

β-TUB

1.796

 

7

α-TUB

2.570

GAPDH

2.077

α-TUB

1.983

α-TUB

2.025

 

8

TBP

2.685

Rpl8

2.090

TBP

2.139

TBP

2.182

 

9

Rps7

3.016

Actin

2.338

Rps7

2.602

Rps7

2.367

Different

1

Rpl8

1.194

18S

0.477

18S

0.659

Rpl8|

0.567

EF1

Drugs

2

α-TUB

1.200

β-TUB

0.637

α-TUB

0.668

  
 

3

18S

1.238

TBP

0.839

TBP

0.758

Actin

0.705

 

4

GAPDH

1.239

GAPDH

0.948

Rpl8

0.785

GAPDH

0.789

 

5

TBP

1.246

Actin

0.980

GAPDH

0.798

α-TUB

0.977

 

6

Actin

1.295

Rpl8

1.057

Actin

0.918

TBP

1.054

 

7

β-TUB

1.483

α-TUB

1.071

β-TUB

1.178

18S

1.113

 

8

EF1

1.507

EF1

1.094

EF1

1.314

β-TUB

1.229

 

9

Rps7

1.800

Rps7

1.184

Rps7

1.646

Rps7

1.356

Different

1

GAPDH

0.604

β-TUB

0.233

GAPDH

0.212

α-TUB|

0.260

β-TUB

Heavy metals

2

α-TUB

0.611

GAPDH

0.269

α-TUB

0.297

  
 

3

β-TUB

0.636

α-TUB

0.338

β-TUB

0.351

GAPDH

0.336

 

4

Rpl8

0.691

Rpl8

0.408

Rpl8

0.423

Rpl8

0.411

 

5

Actin

0.728

Actin

0.462

Actin

0.476

Actin

0.491

 

6

Rps7

0.744

Rps7

0.528

Rps7

0.556

Rps7

0.577

 

7

EF1

0.790

EF1

0.587

EF1

0.607

EF1

0.618

 

8

18S

0.841

TBP

0.613

18S

0.705

18S

0.650

 

9

TBP

1.095

18S

0.655

TBP

1.015

TBP

0.749

Different

1

TBP

1.232

EF1

0.523

EF1

0.059

EF1|

0.272

TBP

Tissues

2

EF1

1.269

TBP

0.558

TBP

0.136

  
 

3

Rpl8

1.306

α-TUB

0.634

Rpl8

0.221

Rpl8

0.428

 

4

Rps7

1.412

Actin

0.749

Rps7

0.401

Rps7

0.639

 

5

GAPDH

1.532

β-TUB

0.760

GAPDH

0.715

GAPDH

0.787

 

6

α-TUB

1.572

Rps7

0.863

β-TUB

1.190

β-TUB

0.932

 

7

β-TUB

1.753

Rpl8

0.887

α-TUB

1.477

α-TUB

1.026

 

8

Actin

2.001

GAPDH

0.957

Actin

1.590

Actin

1.193

 

9

18S

3.729

18S

3.714

18S

3.655

18S

1.756