Fig. 7From: A cecropin-like antimicrobial peptide with anti-inflammatory activity from the black fly salivary glandsEffects of SibaCec on LPS-induced inflammatory response pathways. Western blot of phosphorylation of ERK and p38, and the translocation of the NF-κB p65 subunit from cytoplasm to nucleus in peritoneal macrophages. The cells were incubated with LPS (100 ng/ml) and different concentrations of SibaCec (5, 10 and 20 μg/ml). After incubation for 30 min, the cells were collected, and the cytoplasmic or nuclear proteins were extracted for Western blot analysisBack to article page