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Fig. 1 | Parasites & Vectors

Fig. 1

From: Protective antibody and cytokine responses in mice following immunization with recombinant beta-tubulin and subsequent Trypanosoma evansi challenge

Fig. 1

Heterologous expression of recombinant β-tubulin from T. evansi. a PCR amplification of T. evansi β-tubulin gene. b Induced heterologous expression and purification of recombinant T. evansi β-tubulin protein (50 kDa) shown by SDS-PAGE. Protein molecular weight marker (Lane 1), pre-induction Te-β-tubulin transgenic E. coli lysate (lane 2), induced Te-β-tubulin transgenic E.coli lysate collected 2, 4,or 6 h post-induction (lanes 3-5, respectively) and affinity-purified, renatured rTe-β-tubulin (lane 6). c Identity of the expressed rTe-β-tubulin confirmed by Western blot using anti-His-tag antibody probing induced (4 h) Te-β-tubulin transgenic E. coli culture supernatant (lane 1), pre-induction (lane 2) or induced (4 h) (lane 3) Te-β-tubulin transgenic E. coli lysate

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