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Fig. 1 | Parasites & Vectors

Fig. 1

From: Does extreme asymmetric dominance promote hybridization between Anopheles coluzzii and Anopheles gambiae s.s. in seasonal malaria mosquito communities of West Africa?

Fig. 1

Multiplex PCR for simultaneous sexing and species identification of An. gambiae s.l. larvae. The primers developed for a species identification diagnostic [40] and for amplification of a male Y-chromosome specific sequence [39] were combined in a single optimized PCR reaction. The lanes were: DNA size ladder (SL, 1 and 21); negative control (C-, 2); males (3–5) and females (6–8) An. coluzzii bands, hybrid female bands (9–11), males (12–14) and female An. gambiae s.s. (15–17) bands and An. arabiensis (18–20) female bands. Expected sizes are indicated in base-pairs (bp)

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Parasites & Vectors

ISSN: 1756-3305

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