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Fig. 2 | Parasites & Vectors

Fig. 2

From: Experimental evaluation of sand fly collection and storage methods for the isolation and molecular detection of Phlebotomus-borne viruses

Fig. 2

Gel electrophoresis of RT- and Nested-RT-PCR amplicons of a portion of SFNV complex of Phlebovirus N gene. AS-CDC: alive blood-fed sand flies stored at −80 °C immediately after TOSV infection; DS-CDC: dead blood-fed sand flies recovered after 1 day post infectious blood meal at room temperature; DS-St: dead blood-fed females left at room temperature on sticky papers and stored without EtOH; DS-St + EtOH: dead blood-fed sand flies left at room temperature on sticky papers and then stored with EtOH; TOSV: Toscana Virus; SFNV: Sand fly Fever Naples Virus; N: negative control; M: 100 bp ladder

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