Experimental evaluation of sand fly collection and storage methods for the isolation and molecular detection of Phlebotomus-borne viruses

Table 1 TOSV isolation by CPE in VERO cell cultures from sand flies individually processed

Sandflies collection and storage methods	Day post infection	1^st Experimental infection	2^nd Experimental infection	Total
			Pos/tested (%)
AS-CDC	0	10/10 (100)	10/10 (100)	20/20 (100)
DS-CDC	1	13/13 (100)	13/15 (87)	26/28 (93)
DS-St	1	2/5 (40)	2/5 (40)	4/10 (40)
	2	2/5 (40)	1/5 (20)	3/10 (30)
	3	2/5 (40)	1/5 (20)	3/10 (30)
	4	1/5 (20)	0/5 (0)	1/10 (10)
	6	0/5 (0)	0/5 (0)	0/10 (0)
DS-St + EtOH	1	0/5 (0)	0/5 (0)	0/10 (0)
	2	0/5 (0)	0/5 (0)	0/10 (0)
	3	0/5 (0)	0/5 (0)	0/10 (0)
	4	0/5 (0)	0/5 (0)	0/10 (0)
	6	0/5 (0)	0/5 (0)	0/10 (0)

AS-CDC alive blood-fed sand flies stored at −80 °C immediately after TOSV infection, DS-CDC dead blood-fed sand flies recovered after 1 day post infectious blood meal at room temperature, DS-St dead blood-fed females left at room temperature on sticky papers and stored without EtOH, DS-St + EtOH dead blood-fed sand flies left at room temperature on sticky papers and then stored with EtOH, CPE cytopathic effect

ISSN: 1756-3305