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Table 2 TOSV RNA quantification by qRT-PCR in sand flies individually processed

From: Experimental evaluation of sand fly collection and storage methods for the isolation and molecular detection of Phlebotomus-borne viruses

Sandflies collection and storage methods

Day post infection

1st Experimental infection

2nd Experimental infection

U Mann Whitney Test

Total

Pos/tested (%) mean ± SD

P value

Pos/tested (%)

AS-CDC

0

10/10(100) 3.74 ± 0.34

10/10(100) 3.61 ± 0.54

0.393

20/20 (100)

DS-CDC

1

13/13(100) 3.78 ± 0.71

15/15(100) 3.46 ± 0.60

0.413

28/28 (100)

DS-St

1

5/5(100) 3.07 ± 0.27

5/5(100) 2.60 ± 0.47

  

2

5/5(100) 2.86 ± 1.27

5/5(100) 3.11 ± 0.20

  

3

5/5(100) 3.10 ± 0.54

5/5(100) 2.54 ± 0.33

0.548

50/50 (100)

4

5/5(100) 3.16 ± 0.26

5/5(100) 2.95 ± 0.29

  

6

5/5(100) 3.11 ± 0.70

5/5(100) 3.23 ± 0.10

  

DS-St + EtOH

1

4/5(80) 2.63 ± 0.77

5/5(100) 1.47 ± 0.38

  

2

3/5(60) 2.06 ± 0.02

5/5(100) 1.47 ± 0.64

  

3

5/5(100) 2.29 ± 0.51

5/5(100) 2.33 ± 0.48

0.310

47/50 (94)

4

5/5(100) 2.84 ± 0.76

5/5(100) 2.63 ± 0.69

  

6

5/5(100) 3.01 ± 0.44

5/5(100) 2.65 ± 0.56

  

Total

 

73

75

 

145/148 (98)

  1. SD standard deviation, AS-CDC alive blood-fed sand flies stored at −80 °C immediately after TOSV infection, DS-CDC dead blood-fed sand flies recovered after 1 day post infectious blood meal at room temperature, DS-St dead blood-fed females left at room temperature on sticky papers and stored without EtOH, DS-St + EtOH dead sand flies left at room temperature on sticky papers and then stored with EtOH