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Table 3 TOSV RNA detection by Nested-RT-PCR for SFNV complex of Phlebovirus in sand flies individually processed

From: Experimental evaluation of sand fly collection and storage methods for the isolation and molecular detection of Phlebotomus-borne viruses

Sand flies collection and storage methods

Day post infection

1st Experimental infection

2nd Experimental infection

Total

   

Pos/tested (%)

AS-CDC

0

3/3 (100)

3/3 (100)

6/6 (100)

DS-CDC

1

3/3 (100)

3/3 (100)

6/6 (100)

DS-St

1

5/5 (100)

4/4 (100)

9/9 (100)

 

2

2/3 (67)

4/4 (100)

6/7 (86)

 

3

4/4 (100)

4/4 (100)

8/8 (100)

 

4

3/3 (100)

4/4 (100)

7/7 (100)

 

6

3/3 (100)

4/4 (100)

7/7 (100)

DS-St + EtOH

1

4/5 (80)

3/4 (75)

7/9 (78)

 

2

3/5 (60)

4/4 (100)

7/9 (78)

 

3

5/5 (100)

4/4 (100)

9/9 (100)

 

4

5/5 (100)

4/4 (100)

9/9 (100)

 

6

5/5 (100)

4/4 (100)

9/9 (100)

  1. AS-CDC alive blood-fed sand flies stored at −80 °C immediately after TOSV infection, DS-CDC dead blood-fed sand flies recovered after 1 day post infectious blood meal at room temperature, DS-St dead blood-fed females left at room temperature on sticky papers and stored without EtOH, DS-St + EtOH dead blood-fed sand flies left at room temperature on sticky papers and then stored with EtOH
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