Fig. 2

GR-MF spheroids stimulation by TGF-β and IL-13 a GR-MF were stimulated, in monolayer, with TGF-β (30 and 60 ng/mL) for 20 h. Cells were incubated with 3[H]-thymidine for 4 h. b Detection of α-actin by immunoenzymatic reaction in spheroids. c Representative photomicrography of GR-MF spheroids cultured for 4 days in the presence of TGF-β (30 ng/mL) or IL-13 (40 ng/mL). d Spheres diameters from at least four spheres from each group were measured using the Image Pro Plus program. This graph is representative from three experiments. *P ≤ 0.05 compared with non-stimulated cells