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Fig. 5 | Parasites & Vectors

Fig. 5

From: Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro

Fig. 5

Eotaxin expression by GR-MF a Real time RT-PCR to determine the relative expression of eotaxin by GR-MF stimulated with TGF-β (30 ng/mL) and IL-13 (40 ng/mL). The relative value was obtained in relation to GAPDH expression. b Eotaxin levels measured by ELISA into supernatant of GR-MF stimulated with TGF-β (30 ng/mL) and IL-13 (40 ng/mL). c Immunolocalization of eotaxin in GR-MF. Magnification 600X. d Eotaxin levels measured by ELISA into supernatant of GR-MF spheroids stimulated with TGF-β (30 ng/mL) and IL-13 (40 ng/mL) after 4 days in culture. These graphs are representative of three experiments. *P ≤ 0.05 compared with non-stimulated cells

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