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Fig. 1 | Parasites & Vectors

Fig. 1

From: High-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation (HITS-CLIP) reveals Argonaute-associated microRNAs and targets in Schistosoma japonicum

Fig. 1

Generation of the anti-SjAgo1 antibody. a Schematic representation of the full-length SjAgo1 protein and the recombinant rSjAgo1 protein with Hisx6 at the C-terminus as an affinity purification tag. b Restriction enzyme digestion analysis of the recombinant plasmid pET28a (+)-Ago1-Piwi. Lane 1 is the recombinant plasmid digested with BamH I and Nco I; lane 2 is the control, consisting of the cloned fragment of the SjAgo1 gene. M. DNA ladder. c Coomassie brilliant blue staining of a 12 % SDS-PAGE gel of the total E.coli lysate (1), flow-through (2), and eluted fractions (3 and 4) from the purification of rSjAgo1 by nickel column chromatography. M. prestained protein ladder. d Western blot assay of rSjAgo1. Lane1 and lane 3 are purified rSjAgo1; Lane 2 and lane 4 are crude E.coli lysate. M. prestained protein ladder. e Western blot assay of total extract of S.japonicum with anti-SjAgo serum (1) or pre-immune serum (2). The recombinant rSjAgo1 is indicated by an asterisk

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