Fig. 1
Characterisation of TBEV infection in IDE8 and IRE/CTVM19 cells. a Percentage of E protein-positive tick cells following TBEV infection. IDE8 and IRE/CTVM19 cells were infected with TBEV at MOI 0.1, 1.0 and 5.0 and cells were fixed and immunostained at day 2 p.i. The percentage of E protein-positive cells was calculated. The mean of duplicate cultures is shown. b TBEV production over a 10-day time course in tick cell lines. IDE8 and IRE/CTVM19 cells were infected with TBEV at MOI 5 and virus was titrated by plaque assay on PS cells. The mean PFU/ml of duplicate cultures is shown. The limit of detection was 56 PFU/ml in a 10−1 dilution