Fig. 5

Dynamic cysteine proteases changes in salivary glands of unfed, fed and engorged ticks. Salivary glands were isolated during the different blood feeding statuses and cysteine proteases expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction. Gene expression was calculated using the △Ct method. Elongation factor served as the endogenous control. Data are represented as LS means (n = 4 ticks). Significant difference analysis was compared the results of unfed, fed and engorged ticks (*means P < 0.05, **means P < 0.01, and ***means P < 0.001)