Fig. 6From: Differential sialotranscriptomes of unfed and fed Rhipicephalus haemaphysaloides, with particular regard to differentially expressed genes of cysteine proteasesDynamic cysteine proteases changes in different development of unfed, fed and engorged ticks. Cysteine proteases expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction. Gene expression was calculated using the △Ct method. Elongation factor served as the endogenous control. Data are represented as LS means (n = 4 ticks). Significant difference analysis was compared the results of unfed, fed and engorged ticks (*means P < 0.05, **means P < 0.01, and ***means P < 0.001)Back to article page