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Fig. 4 | Parasites & Vectors

Fig. 4

From: Natural hybrid of Leishmania infantum/L. donovani: development in Phlebotomus tobbi, P. perniciosus and Lutzomyia longipalpis and comparison with non-hybrid strains differing in tissue tropism

Fig. 4

Development of five strains from Leishmania donovani complex in Phlebotomus perniciosus. a: Infection rates and intensities (weak, moderate, heavy) of five strains from L. donovani complex: CUK3 (dermotropic L. infantum), OG-VL (viscerotropic L. infantum), IMT373 (viscerotropic L. infantum), CYPR (dermotropic L. donovani) and GEBRE (viscerotropic L. donovani) in P. pernicicosus examined on days 2 and 8 PBM (post blood meal) using light microscopy. The infection intensities were classified into three categories according to their intensity: heavy (more than 1000 parasites per gut [black]); moderate (500–1000 parasites [grey]) and light (less than 500 parasites [white]). Numbers above the bars indicate the total number of dissected sand fly females. Data are summarised from at least two independent experiments. b: Analysis of the parasite loads by quantitative PCR (qPCR) in P. pernicicosus on day 8 PBM. Thirty to sixty midguts were analysed for each line

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Parasites & Vectors

ISSN: 1756-3305

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