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Fig. 4 | Parasites & Vectors

Fig. 4

From: Acute chagas outbreaks: molecular and biological features of Trypanosoma cruzi isolates, and clinical aspects of acute cases in Santander, Colombia

Fig. 4

Differentiation of T. cruzi epimastigotes. Epimastigotes were cultured in Grace’s medium and the number of epimastigotes and culture medium-derived trypomastigote (cmDT) were evaluated. Figures a, b, and c. Epimastigote numbers after 2, 5, 7, 10 and 12 days of assay. d. Day of maximum numbers of cmDT. e. Maximum percentage of cmDT in total parasites (epimastigotes plus cmDT). Each experiment was evaluated in triplicate and repeated two times

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