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Fig. 2 | Parasites & Vectors

Fig. 2

From: Leucocyte-derived extracellular trap formation significantly contributes to Haemonchus contortus larval entrapment

Fig. 2

Co-localization of exracellular DNA with histones, MPO and NE in H. contortus-triggered NET structures. Co-cultures of bovine PMN with H. contortus L3 were fixed, permeabilized, stained for DNA using Sytox Orange and probed for histones using anti-histone H1, H2A/H2B, H3, H4 monoclonal antibodies, for MPO using anti-MPO and for NE using anti-NE antibodies. a, d, g: Extracellular DNA stained with Sytox Orange (red). b, e, h: MPO, NE and histones staining within NET structures (green), respectively. c, f, i: Overlay of NET-DNA with MPO, NE, and histones respectively. Note that distinct forms of NETs were observed in fluorescence microscopy: sprNET (c, f white arrows), aggNET (f red arrow) diffNET (f yellow arrow) and clasp-like NET (i red arrow). Photomicrographs represent exemplary images of 3 independent experiments

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