Fig. 4

H. contortus-triggered NETosis is independent on the parasite integrity and low pH conditions. a: Bovine PMN were exposed to vital ensheathed larvae, heat-inactivated larvae or parasite-free controls. NET measurement was performed using PicoGreen-derived fluorescence intensities. b: Bovine PMN were exposed to H. contortus L₃ at different pH conditions (pH 2.0–3.5) and NET measurement was performed using Sytox Orange®-derived fluorescence intensities with an anti-histone H1, H2A/H2B, H3, H4 monoclonal antibody, [mouse clone H11-4, 1:1000, 1 h, RT], jointly with a secondary antibody (Alexa Fluor® 488 Goat Anti-Mouse IgG). As positive control, stimulation of PMN by zymosan (1 mg/ml) was used. For negative control, PMN in plain medium were used