|
Antisera at different dilutions
|
OD450 values of antigen at different coating concentrations
|
|---|
|
0.06 μg
|
0.12 μg
|
0.24 μg
|
0.48 μg
|
0.96 μg
|
1.92 μg
|
|---|
|
1:5 (P)
|
1.181
|
1.137
|
1.065
|
1.078
|
1.182
|
1.178
|
|
1:5 (N)
|
0.427
|
0.361
|
0.319
|
0.307
|
0.478
|
0.487
|
|
1:10 (P)
|
0.932
|
0.908
|
0.896
|
0.789
|
0.858
|
0.873
|
|
1:10 (N)
|
0.357
|
0.288
|
0.246
|
0.238
|
0.357
|
0.371
|
|
1:20 (P)
|
0.664
|
0.621
|
0.542
|
0.501
|
0.645
|
0.626
|
|
1:20 (N)
|
0.238
|
0.244
|
0.191
|
0.180
|
0.275
|
0.245
|
|
1:40 (P)
|
0.461
|
0.447
|
0.347
|
0.397
|
0.473
|
0.445
|
|
1:40 (N)
|
0.198
|
0.165
|
0.131
|
0.148
|
0.223
|
0.227
|
|
1:80 (P)
|
0.348
|
0.339
|
0.298
|
0.305
|
0.392
|
0.381
|
|
1:80 (N)
|
0.157
|
0.143
|
0.115
|
0.122
|
0.134
|
0.139
|
- Note: N, positive serum; P, negative serum; The values in bold represent optimum conditions of this indirect ELISA method
