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Table 1 Determination of the coating concentration of protein and serum dilution in optimization of the indirect ELISA method

From: Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2

Antisera at different dilutions OD450 values of antigen at different coating concentrations
0.06 μg 0.12 μg 0.24 μg 0.48 μg 0.96 μg 1.92 μg
1:5 (P) 1.181 1.137 1.065 1.078 1.182 1.178
1:5 (N) 0.427 0.361 0.319 0.307 0.478 0.487
1:10 (P) 0.932 0.908 0.896 0.789 0.858 0.873
1:10 (N) 0.357 0.288 0.246 0.238 0.357 0.371
1:20 (P) 0.664 0.621 0.542 0.501 0.645 0.626
1:20 (N) 0.238 0.244 0.191 0.180 0.275 0.245
1:40 (P) 0.461 0.447 0.347 0.397 0.473 0.445
1:40 (N) 0.198 0.165 0.131 0.148 0.223 0.227
1:80 (P) 0.348 0.339 0.298 0.305 0.392 0.381
1:80 (N) 0.157 0.143 0.115 0.122 0.134 0.139
  1. Note: N, positive serum; P, negative serum; The values in bold represent optimum conditions of this indirect ELISA method