Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2

Parasites & Vectors

Table 1 Determination of the coating concentration of protein and serum dilution in optimization of the indirect ELISA method

Antisera at different dilutions	OD450 values of antigen at different coating concentrations
Antisera at different dilutions	0.06 μg	0.12 μg	0.24 μg	0.48 μg	0.96 μg	1.92 μg
1:5 (P)	1.181	1.137	1.065	1.078	1.182	1.178
1:5 (N)	0.427	0.361	0.319	0.307	0.478	0.487
1:10 (P)	0.932	0.908	0.896	0.789	0.858	0.873
1:10 (N)	0.357	0.288	0.246	0.238	0.357	0.371
1:20 (P)	0.664	0.621	0.542	0.501	0.645	0.626
1:20 (N)	0.238	0.244	0.191	0.180	0.275	0.245
1:40 (P)	0.461	0.447	0.347	0.397	0.473	0.445
1:40 (N)	0.198	0.165	0.131	0.148	0.223	0.227
1:80 (P)	0.348	0.339	0.298	0.305	0.392	0.381
1:80 (N)	0.157	0.143	0.115	0.122	0.134	0.139

Note: N, positive serum; P, negative serum; The values in bold represent optimum conditions of this indirect ELISA method

ISSN: 1756-3305