Fig. 3

Immunoblot analysis demonstrating the pattern of expression of RmAQP2 in different tick life stages and tissues. Two bands at approximately 30 and 50 KDa were detected in salivary glands (SG) of partially engorged females. No protein band was detected in larvae, nymphs or guts of partially engorged females. Hybridoma supernatant 147-677-11 was used at a 1:3 dilution. Anti-mouse HRP conjugates were used at a 1:5,000 dilution. Assays were developed using chemiluminescent HRP detection reagents