Fig. 5

Secreted salivary protein components are localized within SG cells and secretory cavities. Confocal single slice images of adult An. stephensi SG lobes of females (a) and males (b) imaged by DIC (not shown; lobes are outlined thinly in white), stained with DAPI (nuclei, blue) and antibodies against the mosquito SG protein products SG6 (red) and AAPP (green). Dashed boxes indicate regions magnified in images to the right. SG6 is observed as punctate cytosolic foci in all female lobes and male SGs (e.g. Axiii, white arrow). Accumulation of SG6 was seen in female PL lobe secretory cavities (Avii, arrow). AAPP staining is seen weakly throughout the cell body in female PL and male lobes, with basolateral enrichment in the DL lobe, and basal enrichment in the PL and M lobes (Ax, xvi, yellow arrows). Both proteins are seen in the secretory cavities of male SGs (Biii, arrow) and SG6 is seen in the secretory cavity of the female PL lobe (vi-ix). AAPP signal is also observed in the duct and perinuclearly in males (Bvi). Scale bar lengths are: 50 microns—Ai, Avi, Axii, Bi; 20 microns—Aii, Avii, Axiii, Biii