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Table 1 Results of molecular analyses of 596 ticks collected in 2007 in Hungary

From: Diversity of Haemaphysalis-associated piroplasms of ruminants in Central-Eastern Europe, Hungary

Haemaphysalis species

Tick stage or sex

Number of ticks per pool

PCR positive/all pools

Results of sequencing (length, % identity, sample number)

Location in Hungarya

Reference sequence

Accession number of sequence in this study (name of isolate)

H. inermis

male

5

7/24

Babesia crassa (410 bp, 98.6 %, 4×)

B, P, S

KF791205

KT725848 (Bcr-Hu1)

female

5

12/40

Babesia crassa (410 bp, 98.6 %, 6×)

B, P, S

KF791205

KT725848 (Bcr-Hu1)

H. concinna

male

3

10/29

Babesia sp. Kh-Hc222 (409 bp, 99.8 %, 1×)

P

KJ486568

KT725849 (BKh-Hu1)

Babesia sp. Irk-Hc133 (409 bp, 100 %, 2×)

B, P, A, C

KJ486563

KT725852 (BIrk-Hu1)

Theileria capreoli (439 bp, 99.8 %, 2×)

B, P

AY726008

KT725850 (Tc-Hu1)

Theileria sp. ZS TO4b (440 bp, 100 %, 1×)

P

DQ520836

KT725851 (TZSTO4-Hu1)

female

3

5/16

Babesia motasi (403 bp, 99.3 %, 1×)

B

AY260179

KT725853 (Bmo-Hu1)

Babesia sp. Irk-Hc133 (409 bp, 100 %, 1×)

B, P, A, C

KJ486563

KT725852 (BIrk-Hu1)

Theileria sp. ZS TO4 (440 bp, 100 %, 1×)

P

DQ520836

KT725851 (TZSTO4-Hu1)

nymph

10

7/7

Babesia sp. Irk-Hc133 (409 bp, 100 %, 1×)

B, P, A, C

KJ486563

KT725852 (BIrk-Hu1)

Theileria capreoli (439 bp, 99.8 %, 1×)

B, P

AY726008

KT725850 (Tc-Hu1)

Theileria sp. ZS TO4 (440 bp, 100 %, 1×)

P

DQ520836

KT725851 (TZSTO4-Hu1)

larva

52

0/1

-

 

-

-

H. punctata

male

5

1/5

Theileria sp. OT3 (160 bp, 100 %, 1×)

P

KF470868

KT725854 (TOT3-Hu1)

female

3

1/3

Theileria sp. OT3 (160 bp, 100 %, 1×)

V

KF470868

KT725854 (TOT3-Hu1)

nymph

1

0/1

-

 

-

-

  1. a Abbreviations: B Börzsöny Mountains, P Pilis Mountains, S Szekszárd Hills, V Visegrád Mountains, A Ásotthalom, C Cserhát Mountains
  2. bNote that Theileria sp. ZS TO4 and T. capreoli are 99.5 % identical, but for comparability with relevant literature data and because of their separate clustering on the phylogentic tree (Fig. 1) both names are maintained in the table and in the text