Fig. 2
From: HisAK70: progress towards a vaccine against different forms of leishmaniosis
Expression of HisAK70 in CHO-K1 cells after transfection. a-b HisAK70 protein expression was detected from CHO-K1 transfected cells using specific anti-A2 and anti-E Tag antibodies. Lane 1, total protein extract from empty vector-transfected cells (pVAX1-asd); lane 2, cells transfected with pVAX1::HISAK70-asd; and lane 3, extract from non-transfected CHO-K1 cells. Proteins were separated on a 10 % SDS-PAGE gel. The positions of molecular mass markers are indicated on the right. HisAK70 (152 kDa) and the control protein loading, β-Tubulin (52 kDa), are highlighted by arrows, on the left. Data of one representative out of three independents are given. Relevant portions of each blot are shown