Fig. 4

HisAK70 vaccinated mice develop efficient sterile granulomas. Percentage of hepatic granuloma maturation and representative granulomas from H&E stained liver sections at day 42 p.i. in (a) PBS, (b) empty vector and (c) HisAK70 vaccinated mice. Control mice show high amount of immature granulomas, whereas HisaK70 vaccinated mice show well-developed mature and sterile granulomas. Images were acquired at the indicated magnifications and arrows indicate the presence of amastigotes in the granulomas. All data are presented as the representative mean from each experimental group of mice. Asterisks indicate P < 0.01 with respect to control mice. IM, immature granuloma; M, mature granuloma; Sterile, parasite-free granuloma