Fig. 2

Identification of extracellular expression of yCsCBs by Western Blotting. a Cell culture medium of yCsCBs (yCsCB1, yCsCB2, yCsCB3 and yCsCB4) was probed with mouse anti-His antibody (1: 500). b Cell culture medium of yCsCB1 was probed with mouse anti-c-Myc antibody (1: 500). c Cell culture medium of yCsCB1 was probed with rat anti-CsCB1 antibody (1: 800). Lanes 1–5 indicated ammonium sulfate precipitate, 72-h culture medium, 96-h culture medium, 120-h culture medium and 144-h culture medium, respectively