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Fig. 4 | Parasites & Vectors

Fig. 4

From: Identification and characterization of Clonorchis sinensis cathepsin B proteases in the pathogenesis of clonorchiasis

Fig. 4

Host proteins degradation by yCsCBs. The assays were performed in a 200 μl mixture containing Na3PO4 (100 mM, pH 5.5), EDTANa2 (1 mM), DTT (10 mM), yCsCBs (20 μM) and various host proteins (1 mg). The reactions were terminated by adding a reducing sample buffer and analyzed by SDS-PAGE. a Gelatin hydrolysis assay using yCsCBs versus eCsCBs. b Degradation assay using bovine serum albumin (BSA) as the substrate. c Degradation assay using human serum albumin (HSA) as the substrate. d Degradation assay using human fibronectin (HFN) as the substrate. e Degradation assay using human hemoglobin (Hb) as the substrate. f Degradation assay using human IgG as the substrate. The molecular mass of host proteins was indicated

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