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Fig. 7 | Parasites & Vectors

Fig. 7

From: Identification and characterization of Clonorchis sinensis cathepsin B proteases in the pathogenesis of clonorchiasis

Fig. 7

The effect of yCsCB4 on human MHCC-97H and RBE cells. a Cell proliferation level measured by CCK-8 assay. b Cell cycle analysis by flow cytometry, the percentage of cells in the G2/S period was analyzed and quantified using FlowJo software. c, d Cell migration of MHCC-97H cells shown by wound-healing assay. Cells were observed using light microscope under 10X objective. e, f Cell migration of RBE cells shown by wound-healing assays; cells were observed using a light microscope under 10X objective. Generally, MHCC-97H cells or RBE cells were incubated with 1 μg/ml of yCsCB4 or PBS for 24 h and assays were performed. Assays were performed in triplicate. Relative cell migration level was calculated by normalizing to cell migration level at 0 h. *P < 0.05, ***P < 0.001, compared to PBS control

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