Table 1 Primer sequences and cycling conditions used to amplify K13-propeller gene of Plasmodium falciparum isolates ^a

K13-propeller (P)

F: 5′-cggagtgaccaaatctggga-3′

R: 5′-gggaatctggtggtaacagc-3′

95 °C 5 min/[95 °C 30 s, 60 °C 90 s, 72 °C 90 s] × 40 cycles, 72 °C 10 min

2096

[12]

K13-propeller (S)

F: 5′-gccaagctgccattcatttg-3′

R: 5′-gccttgttgaaagaagcaga-3′

95 °C 5 min/[95 °C 30 s, 60 °C 90 s, 72 °C 90 s] × 40 cycles, 72 °C 10 min

848

[12]