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Table 1 Primer sequences and cycling conditions used to amplify K13-propeller gene of Plasmodium falciparum isolates a

From: Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy

Genesb Primersc PCR cycling conditions Product size Reference
K13-propeller (P) F: 5′-cggagtgaccaaatctggga-3′
R: 5′-gggaatctggtggtaacagc-3′
95 °C 5 min/[95 °C 30 s, 60 °C 90 s, 72 °C 90 s] × 40 cycles, 72 °C 10 min 2096 [12]
K13-propeller (S) F: 5′-gccaagctgccattcatttg-3′
R: 5′-gccttgttgaaagaagcaga-3′
95 °C 5 min/[95 °C 30 s, 60 °C 90 s, 72 °C 90 s] × 40 cycles, 72 °C 10 min 848 [12]
  1. a P. falciparum isolates from Grande Comore Island, Union of Comoros
  2. b P Primary PCR reaction, S Secondary PCR reaction
  3. c F Forward primer, R Reverse primer