Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy

Table 2 Prevalence of K13-propeller mutations in Plasmodium falciparum isolates^a

Areas	Mutations	Amino acid and genetic changes^b	Number of isolates (%)^c
Areas	Mutations	Amino acid and genetic changes^b	2006–2007 (n = 95)	2013–2014 (n = 85)
Mitsoudje Center Hospital	Synonymous	R471R (CGT → CGC)	0 (0)	1 (1.2)
	Synonymous	G538G (GGT → GGA)	1 (1.1)	2 (2.4)
	Non-synonymous	D464H (GAT → CAT)	0 (0)	1 (1.2)
		D464Y (GAT → TAT)	0 (0)	1 (1.2)
		L488S (TTG → TCG)	0 (0)	1 (1.2)
		I526M (ATA → ATG)	0 (0)	1 (1.2)
		A578S (GCT → TCT)	0 (0)	2 (2.4)
		D584E (GAT → GAA)	1 (1.1)	1 (1.2)
National Malaria Center	Synonymous	Y500Y (TAT → TAC)	0 (0)	1 (1.2)
	Non-synonymous	D464H (GAT → CAT)	0 (0)	1 (1.2)
		S477Y (TCT → TAT)	0 (0)	1 (1.2)
		I526M (ATA → ATG)	0 (0)	1 (1.2)
		A578S (GCT → TCT)	0 (0)	2 (2.4)
Mitsamiouli Center Hospital	Synonymous	Y500Y (TAT → TAC)	0 (0)	1 (1.2)
	Non-synonymous	D464Y (GAT → TAT)	0 (0)	2 (2.4)
		S477Y (TCT → TAT)	1 (1.1)	1 (1.2)
		A504T (GCT → ACT)	0 (0)	1 (1.2)
		D584E (GAT → GAA)	0 (0)	2 (2.4)

^aIsolates collected from 2006 to 2007 and 2013 to 2014 along Grande Comore Island of Comoros
^bThe mutated amino acids and nucleotides are indicated in bold type
^cStatistically significant differences for comparison with isolates circulating in 2006–2007 from Grande Comore Island (*P < 0.05) using Mann–Whitney U test

ISSN: 1756-3305