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Fig. 6 | Parasites & Vectors

Fig. 6

From: New insights into the evolution of the Trypanosoma cruzi clade provided by a new trypanosome species tightly linked to Neotropical Pteronotus bats and related to an Australian lineage of trypanosomes

Fig. 6

Pteronotus parnellii and developmental forms of T. wauwau: Light microscopy of Giemsa-stained forms: A, trypomastigotes in bat blood smear. B, flagellates co-cultivated with Hi-5 insect cells: Supernatants of early cultures showing small and rounded division forms (a, b, c), multiple fission forms united by the posterior extremity exhibiting various nuclei, kinetoplasts and flagella (a, c), rosettes of epimastigotes attached by the flagella (b), epimastigotes largely varying in shape and size (b, d-f), log-phase regular epimastigote that multiply by binary fission (d), small trypomastigotes with terminal kinetoplast of stationary cultures (g). C, Epimastigotes (a, b) and trypomastigotes (b) with noticeable undulant membrane, and small trypomastigotes (a) in the supernatant of LLC-MK2 mammalian cells at 37 °C. Trypomastigotes are indicated by black stars. Nucleus (N); Kinetoplast (K); Flagellum (F), Undulant Membrane (UM). Scale bars: 10 μm

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