Fig. 2
Expression profiles of genes involved in immune response in the liver of wild-type mice chronically infected with different strains of L. infantum. The relative expression of Arg1 (a), Nos2 (b), Il-10 (c), Il-12p40 (d), Ifng (e) and Tnf (f) in the liver of wild-type mice after 60 days of infection with L. infantum ITMAP-263, IMT-151 or IMT-202 strains was measured by qRT-PCR. Each sample was normalized to Hprt, calculated by the comparative CT method (2-ΔΔCT). The expression was further normalized to expression levels in naïve mice’s liver. Mean and SD of each group are shown (N = 4–6). One-way ANOVA with post hocTukey test was performed (*p < 0.05; **p < 0.01; ***p < 0.001, versus non-infected control)