Table 1 Hosts analyzed within the scope of this study between 2011–2013, comparative prevalence of Hoferellus spp. by species and locality as revealed by light microscopy and by single/duplex PCR screening (SSU, ITS) of kidney, ureters and/or urinary bladder and sequence data obtained in this study. All sampled cyprinids originated from the Czech Republic; Gnathonemus petersii originated from pet shop (imported from Nigeria); frogs (Hyperolius spp.) were sampled separately in 2010 in Kenya
Hoferellus species | Host | Location | Coordinates | Prevalence | Sequences length and GenBank Acc. No. | ||||
|---|---|---|---|---|---|---|---|---|---|
Microscopy (Kid, Ure + UB) | PCR (Kid, Ure + UB; Total) | Partial SSU | ITS region | ||||||
Hoferellus carassii | Carassius auratus (n = 114) | Chřešťovice | 49°19’25.32“N; 14°17’10.68”E | 14 % (12/86), 33,3 % (23/69) | 16.3 % (7/43), 51.5 % (17/33); 51.1 % (23/45) | 1969 bp (KU141399) | - | ||
Bavorov | 49° 7’17.58“N; 14° 4’42.13”E | 0 % (0/14), 14,3 % (1/7) | 7.7 % (1/13), 2/3; 23.1 % (3/13) | - | - | ||||
Jihlava | 49°23’56.32“N; 15°33’33.33”E | 30 % (3/10), 50 % (3/6) | 50 % (4/8), 2/3; 50 % (4/8) | - | 799 bp (KU141423) | ||||
Tourov | 49°07’22“N; 14°02’04”E | 0/4, - | 0/4, -; 0/4 | - | - | ||||
Carassius gibelio (n = 12) | Jihlava | 49°23’56.32“N; 15°33’33.33”E | 0 % (0/12), 58.3 % (7/12) | 0/2, 3/3; 3/3 | 2060 bp (KU141400) | - | |||
H. cyprini PCR (Kid, Ure + UB; Total) | Hoferellus sp. PCR (Kid, Ure + UB; Total) | PCR total mixed infections | |||||||
Hoferellus cyprini & Hoferellus sp. | Cyprinus carpio (n = 131) | Jindřichův Hradec | 49° 9’50.10“N; 15°11’42.20”E | 13.8 % (8/58), 22.4 % (11/49) | 42.85 % (3/7), 60 % (6/10); 53.3 % (8/15) | 7.7 % (1/13), 27.3 % (3/11); 19.1 % (4/21) | 25 % (3/12) | 782 bp (KU141405) | 638 bp (KU141412) 638 bp (KU141413) 648 bp (KU141411) 652 bp (KU141410) |
Chřešťovice | 49°19’25.32“N; 14°17’10.68”E | 13.5 %(7/52), 2.6 % (1/38) | 44.4 % (4/9), 0/7; 26.7 % (4/15) | 12.12 % (4/33), 5 % (1/20); 11.8 % (4/34) | 13.3 % (2/15) | 2046 bp (KU141401) | 796 bp (KU141406) 784 bp (KU141421) 785 bp (KU141417) 790 bp (KU141418) 784 bp (KU141422) | ||
Třeboň | 48°59’57.89“N; 14°46’17.14”E | 14.3 % (1/7), 14,3 % (1/7) | 1/1, 50 % (3/6); 50 % (3/6) | 20 % (1/5), 50 % (3/6); 28.6 % (2/7) | 16.6 % (1/6) | 796 bp (KU141403) | 625 bp (KU141414) | ||
Nakolice, Nové Hrady | 48°47’38.68“N; 14°49’53.82”E | 20 % (1/5), 20 % (1/5) | 0/2, 60 % (3/5); 60 % (3/5) | 0/5, 0/5; 20 % (1/5) | 20 % (1/5) | 854 bp (KU141404) | 605 bp (KU141419) 606 bp (KU141420) 796 bp (KU141407) | ||
Fish market (České Budějovice) | 0/4, 0/4 | 0/2, -; 0/2 | 2/4, -; 2/4 | 0/2 | - | - | |||
Bavorov | 49° 7’17.58“N; 14° 4’42.13”E | 1/3, 0/1 | 1/1, -; 1/2 | 0/2, -; 0/2 | 0/2 | - | - | ||
Jihlava | 49°23’56.32“N; 15°33’33.33”E | 0/2, 1/2 | -, 1/1; 1/2 | -, 1/1; 1/2 | 1/2 | 1995 bp (KU141402) | 798 bp (KU141408) 798 bp (KU141416) 625 bp (KU141409) 797 bp (KU141415) | ||
Hoferellus gnathonemi sp. n. | Gnathonemus petersii (n = 10) | Petshop | NA | 10 % (1/10) (kidney) | 10 % (1/10) (kidney) | 2162 bp (KU141398) | . | ||
Hoferellus anurae | Hyperolius kivuensis (n = 7) | Kakamega, Kenya | 0°20’56“N, 34°51’55.9”E | 57 % (4/7) | - | 964 bp (KU141397) | - | ||
Hyperolius viridiflavus (n = 10) | Kakamega, Kenya | 0°20’56“N, 34°51’55.9”E | 40 % (4/10) | - | - | - | |||