Species complexes and phylogenetic lineages of Hoferellus (Myxozoa, Cnidaria) including revision of the genus: A problematic case for taxonomy

Table 2 Name of primers, ribosomal gene target region, primers’ sequence, amplicon size base pairs, PCR conditions, primers used for sequencing and reference

Primer name	Target rDNA	Sequence 5’-3’	Amplicon size (bp)	Annealing temperature (°C)	PCR round	Sequencing	Author
Erib1	SSU	ACCTGGTTGATCCTGCCAG	≈1900	60	1^st	Yes	[33]
Erib10	SSU	CTTCCGCAGGTTCACCTACGG	≈1900	60	1^st	Yes	[33]
MyxospecF	SSU	TTCTGCCCTATCAACTWGTTG	≈900	52	Nested	Yes	[2]
MyxospecR	SSU	GGTTTCNCDGRGGGMCCAAC	≈900	52	Nested	Yes	[2]
MyxGP2F	SSU	WTGGATAACCGTGGGAAA	≈800	58	Nested	Yes	[34]
Act1R	SSU	AATTTCACCTCTCGCTGCCA	≈800	58	Nested	Yes	[35]
HofcarasF	SSU	GTGTTCTCACGAATGTGTAT	≈300	54	Duplex	No	Present study
HofcarasR	SSU	AACCTATAAGGCTATTATCTG	≈300			No	Present study
HofK41F	SSU	TTGTGTATATTATGTAATGTATTG	≈900			No	Present study
HofK41R	SSU	CATCTTGTTACCAAAATAAC	≈900			No	Present study
HofK48F	SSU	ACGTATGTGTGTTATAATGTGTATG	≈1400	56	Single	No	Present study
HofK48R	SSU	TTTGTTGCCAAAACAACCAC	≈1400	56		No	Present study
HofSSUend	SSU	GTGTACTTCATAAAAGTACGC	≈700	60	Single	Yes	Present study
ITS-Zschok-Rev	LSU	GATTCTCATAGTAACTGCGAGTG	≈700	60	Single	Yes	[36]
HofK102R	ITS	GCACCACAAAAACATTACTT	≈200 (with HofSSUend)	55	Single	No	Present study
HofK107R	ITS	CATGCACCACACAAATTAT	≈200 (with HofSSUend)	55	Single	No	Present study

ISSN: 1756-3305