Table 1 Primers used for amplification of TgGlo1 gene and generation of point mutations of TgGlo1
Gene | Primer | Sequence |
|---|---|---|
TgGlo1 | Forward | 5′-GGGGAATTCATGTCCAGAAGTTTGCCC-3′ |
Reverse | 5′-GGGAAGCTTTTACTTCTTTGAAAGGAACG-3′ | |
E92Q | Forward | 5′-GGGACGTGCTTGCAACTCACTCACAATC-3′ |
Reverse | 5′-GATTGTGAGTGAGTTGCAAGCACGTCCC-3′ | |
E166Q | Forward | 5′-GGATACTGGATACAATTGGTTTCTCGG-3′ |
Reverse | 5′-CCGAGAAACCAATTGTATCCAGTATCC-3′ | |
R188E | Forward | 5′-CAGACGATGATTGAAATCAAAGATCCG-3′ |
Reverse | 5′-CGGATCTTTGATCCGAATCATCGTCTG-3′ | |
E251Q | Forward | 5′-CAGCCTGTATTACAACTCACACACAATCAC-3′ |
Reverse | 5′-GTGATTGTGTGTGAGTTGTAATACAGGCTG-3′ | |
E324Q | Forward | 5′-GGATATAGCATCCAACTGATTCAG-3′ |
Reverse | 5′-CTGAATCAGTTGGATGCTATATCC-3′ |