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Table 1 Primers used for amplification of TgGlo1 gene and generation of point mutations of TgGlo1

From: Characterization of Toxoplasma gondii glyoxalase 1 and evaluation of inhibitory effects of curcumin on the enzyme and parasite cultures

Gene Primer Sequence
TgGlo1 Forward 5′-GGGGAATTCATGTCCAGAAGTTTGCCC-3′
  Reverse 5′-GGGAAGCTTTTACTTCTTTGAAAGGAACG-3′
E92Q Forward 5′-GGGACGTGCTTGCAACTCACTCACAATC-3′
  Reverse 5′-GATTGTGAGTGAGTTGCAAGCACGTCCC-3′
E166Q Forward 5′-GGATACTGGATACAATTGGTTTCTCGG-3′
  Reverse 5′-CCGAGAAACCAATTGTATCCAGTATCC-3′
R188E Forward 5′-CAGACGATGATTGAAATCAAAGATCCG-3′
  Reverse 5′-CGGATCTTTGATCCGAATCATCGTCTG-3′
E251Q Forward 5′-CAGCCTGTATTACAACTCACACACAATCAC-3′
  Reverse 5′-GTGATTGTGTGTGAGTTGTAATACAGGCTG-3′
E324Q Forward 5′-GGATATAGCATCCAACTGATTCAG-3′
  Reverse 5′-CTGAATCAGTTGGATGCTATATCC-3′