Characterization of Toxoplasma gondii glyoxalase 1 and evaluation of inhibitory effects of curcumin on the enzyme and parasite cultures

Table 1 Primers used for amplification of TgGlo1 gene and generation of point mutations of TgGlo1

Gene	Primer	Sequence
TgGlo1	Forward	5′-GGGGAATTCATGTCCAGAAGTTTGCCC-3′
	Reverse	5′-GGGAAGCTTTTACTTCTTTGAAAGGAACG-3′
E92Q	Forward	5′-GGGACGTGCTTGCAACTCACTCACAATC-3′
	Reverse	5′-GATTGTGAGTGAGTTGCAAGCACGTCCC-3′
E166Q	Forward	5′-GGATACTGGATACAATTGGTTTCTCGG-3′
	Reverse	5′-CCGAGAAACCAATTGTATCCAGTATCC-3′
R188E	Forward	5′-CAGACGATGATTGAAATCAAAGATCCG-3′
	Reverse	5′-CGGATCTTTGATCCGAATCATCGTCTG-3′
E251Q	Forward	5′-CAGCCTGTATTACAACTCACACACAATCAC-3′
	Reverse	5′-GTGATTGTGTGTGAGTTGTAATACAGGCTG-3′
E324Q	Forward	5′-GGATATAGCATCCAACTGATTCAG-3′
	Reverse	5′-CTGAATCAGTTGGATGCTATATCC-3′

ISSN: 1756-3305