Table 2 Culicoides imicola microsatellite primer sequences developed and assessed in this study
From: Delineation of the population genetic structure of Culicoides imicola in East and South Africa
Locus name | Left primer | Right primer | NA | Size range | Motif type | Probe DB |
|---|---|---|---|---|---|---|
F1L88 | GTCGGTTGGTGTGTGTCATC | ACGACGACATTATTGACAGCAG | 16 | 180–286 | (GT)^10 | Pr032368558 |
F2629 | ACTCTCAAGGTTTCCGCTG | GACGGCAAACAAAACATGCC | 20 | 130–242 | (GT)^9 | Pr032368559 |
F2KK2 | ACGTGGTACTCAAAGGCAG | CCATGTGATACAGCTTGCGG | 21 | 166–228 | (GT)^9 | Pr032368560 |
F3JMT | CCGATAGTTGTTGTCGTTCATTC | GTATGAGACTCGGTTTGCAC | 10 | 212–274 | (GT)^8 | Pr032368561 |
F7ENT | CTGCCTTTTCCACCTCCAC | ATGCCAGAGTGAAAGCGAC | 12 | 94–198 | (AC)^9 | Pr032368562 |
F9RDNb | AACAAAACACAGCCGCGAG | ATCAGCCAGTCCGCATAAG | 16 | 134–246 | (AC)^9 | Pr032368563 |
FHNDU | ACGGGTCCGTGTATTTGTTG | GACGAGATACGGGCGAGAG | 14 | 172–198 | (AC)^8 | Pr032368564 |
FIGO0a | CAGCAATAAATTGTGTGTCATAACC | GCTTCTCACTCTCCAAACATCTG | 15 | 184–226 | (GT)^8 | Pr032368565 |
FJEAX | TCACGCCTGAACATGGGAG | AACAACAACAGAGGCAGGC | 11 | 185–210 | (GT)^8 | Pr032368566 |
FRB3B | TCCAGCCATCGTCTTTCAG | GGGTGTGTGTAAACTCTATTGTAGC | 15 | 198–234 | (AG)^8 | Pr032368567 |
FYCEH | CGCCACGCCATTTATCGTC | ACTGACAGCTTCCTCTCGC | 26 | 108–248 | (AC)^12 | Pr032368568 |
G1OH4 | TCTCCCAGAAGCGTTTTGC | GTCGTCGTTCTGCCTTGC | 11 | 98–268 | (AC)^8 | Pr032368569 |