Fig. 2

The UNE region is required for the mitochondrial localization of LmABCB3. a Subcellular localization of LmABCB3. Representative picture of L. major promastigotes expressing LmABCB3-GFP or LmABCB3_∆UNE-GFP (GFP) were incubated at 28 °C with 50 nM of Mitotracker Red (Mit-Red) for 30 min at 4 °C. Nomarsky images are shown in the inset. Scale bar: 1 μm. The figure shows a representative parasite of a total population of parasites with a similar fluorescence pattern. b-c Quantitative colocalization analysis. Pearson’s coefficient (b) was calculated for each individual deconvolved image. Mander’s coefficients (c) were calculated to define the M1 and M2 as the proportion of pixels in one channel (M1 = green, GFP) that overlap with some signal in other channel (M2 = red, Mit-Red). Both coefficients were assessed using “JACoP”, a colocalization plugin available in Fiji software. Full: LmABCB3-GFP; ∆UNE: LmABCB3_∆UNE-GFP. ** p < 0.001