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Fig. 4 | Parasites & Vectors

Fig. 4

From: LmABCB3, an atypical mitochondrial ABC transporter essential for Leishmania major virulence, acts in heme and cytosolic iron/sulfur clusters biogenesis

Fig. 4

LmABCB3 is required for the intracellular replication of amastigotes. a. Infection of macrophages by control and LmABCB3 ±/− parasites. Representative picture of the infection of THP-1 macrophages with control and LmABCB3 +/− stationary-phase L. major promastigotes, performed as described in Methods. At the indicated points, cells were fixed and DAPI (blue) stained. The macrophages nuclei (mn) and the kinetoplast and nuclei of intracellular amastigotes (arrowhead) are indicated. Scale bar: 5 um. b LmABCB3 +/− parasites have reduced ability to infect macrophages. THP-1 macrophages were infected with control and LmABCB3 +/−promastigotes as above described and the percentage of infected macrophages (n = 300 macrophages/group) was calculated. The results shown are the means ± SEM of three independent experiments performed in duplicated. **p <0.0002. c LmABCB3 +/−parasites are unable to replicate as intracellular amastigotes. THP-1 macrophages were infected with control and LmABCB3 +/− promastigotes as above described and the average number of intracellular amastigotes per infected macrophage was calculated at the indicated time points. The results shown are the means ± SEM. **p <0.0009

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