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Table 1 Genetic diversity at microsatellite loci of sibling species E at six sampling sites

From: Three sympatric clusters of the malaria vector Anopheles culicifacies E (Diptera: Culicidae) detected in Sri Lanka

Locus

Locations

  

Anu

Mon

Tha

Kan

Kat

Nik

Pooled population

 

N

29

33

32

26

31

42

193

AcA59

A

8

11

11

10

9

9

14

 

Rs

7.896

10.686

10.432

10.000

8.829

7.901

9.830

 

F IS

0.232*

0.246*

0.085*

0.303*

−0.040*

0.100*

0.177*

 

r

0.1204

0.1311

0.0356

0.1662

−0.0273

0.0458

0.1204

 

H e

0.75983

0.88112

0.88641

0.82278

0.83818

0.76592

0.85555

 

H o

0.58621

0.66667

0.81250

0.57692

0.87097

0.69048

0.70466

AcAVIB213

A

7

8

8

9

5

7

12

 

Rs

6.688

7.695

7.582

9.000

4.835

6.282

7.785

 

F IS

0.059*

0.202

0.072

0.332

0.048

−0.026

0.136*

 

r

0.0213

0.1034

0.029

0.1865

0.0159

−0.0187

0.0213

 

H e

0.62250

0.68159

0.57192

0.74359

0.47382

0.58032

0.62312

 

H o

0.58621

0.54545

0.53125

0.50000

0.45161

0.59524

0.53886

AcAIIB5

A

4

4

5

5

2

5

6

 

Rs

3.999

3.958

5.000

5.000

2.000

4.999

4.963

 

F IS

−0.387*

0.660*

0.326*

0.201*

−0.176

0.387*

0.319*

 

r

−0.1672

0.4824

0.1847

0.1001

−0.0877

0.2318

−0.1672

 

H e

0.65094

0.61678

0.69196

0.76697

0.27499

0.77281

0.71499

 

H o

0.89655

0.21212

0.46875

0.61538

0.32258

0.47619

0.48705

AcAVb93

A

5

5

6

7

4

6

9

 

Rs

4.887

4.998

5.962

7.000

3.839

5.853

6.522

 

F IS

0.345*

−0.285

0.120*

0.096*

−0.426

−0.008*

0.047*

 

r

0.1972

−0.1302

0.0548

0.04

−0.1801

−0.0101

0.1972

 

H e

0.62795

0.56830

0.67312

0.76471

0.56954

0.73207

0.70161

 

H o

0.41379

0.72727

0.59375

0.69231

0.80645

0.73810

0.66839

AcA36

A

6

4

5

5

2

6

8

 

Rs

5.990

4.000

4.807

5.000

2.000

5.217

5.664

 

F IS

0.077

0.149

−0.369

0.086

−0.429

−0.071

−0.038

 

r

0.0306

0.0718

−0.1606

0.0342

−0.181

−0.0398

0.0306

 

H e

0.67151

0.67506

0.64286

0.67195

0.43205

0.46730

0.60402

 

H o

0.62069

0.57576

0.87500

0.61538

0.61290

0.50000

0.62694

All Loci

A

6

6.4

7

7.2

4.4

6.6

9.8

 

Rs

5.892

6.267

6.756

7.2

4.299

6.24

6.953

 

F IS

0.070

0.206

0.054

0.207

−0.188

0.097

0.135

 

r

0.0345

0.1002

0.0444

0.0825

0.0666

0.0233

0.0345

 

H e avg

0.66655

0.68457

0.69325

0.75400

0.51772

0.66368

0.69986

 

H o avg

0.62069

0.54545

0.65625

0.60000

0.61290

0.60000

0.60519

  1. N- number of samples, A = number of alleles, F IS – inbreeding coefficient, Rs – allele richness, r- null allele frequency, H e – expected heterozygosity, H o – observed heterozygosity, All loci, all samples – mean values over loci and populations. Probability test against HWE * p < 0.006 after Bonferroni correction for the pooled population and p < 0.001 for the sampling sites. Anu-Anuradhapura, Mon-Monaragala, Tha-Thanamalwila, Kan-Kandy, Kat-Kataragama, Nik-Nikaweratiya